![]() (39) The S1 domain is characterized by an N-terminal domain (NTD) and a C-terminal domain (CTD) ( Figure 3). Each of the three monomeric units are formed by two functional groups, the S1 subunit which is responsible for host recognition and the S2 subunit which guides host–guest membrane fusion. ![]() (25,39) In its functional form, S protein assembles as a homotrimer. Host–guest recognition is virus-specific, and the specificity and selectivity of this process determine both (i) virus tropism and (ii) pathogenesis. As has been observed for other viruses of the Coronaviridae family, (47) it mediates virus entry by contacting specific host-receptors located on the surface of the cell. The S glycoprotein is a structural transmembrane protein of about 1200–1400 amino acid residues per monomer located on the outer envelope of the virion. Remarkably, the N protein is highly immunogenic and is being considered as a potential vaccine target and for the development of COVID-19 diagnostic methods. The success in the development of compounds that interfere with N proteins of other CoVs, such as the recent discovery of stabilizers of the protein–protein interaction of MERS-CoV N protein, (44) reinforces the potential of the N protein as druggable target for SARS-CoV-2 infection. These important structural findings will significantly stimulate the drug discovery of ligands focused on this appealing target to block coronavirus replication and transcription. (41−43) The crystal structure of SARS-CoV-2 nucleocapsid N-terminal domain has been solved (PDB code: 6M3M), (37) showing an overall similarity with the same domain from other CoVs, although the surface electrostatic potential showed a specific distribution. Previous studies revealed that the N-terminal domain is responsible for RNA binding, the C-terminal for oligomerization, and the Ser/Arg-rich linker for primary phosphorylation. (40) This major CoV protein contains two highly conserved domains, an N-terminal RNA-binding domain and a C-terminal dimerization domain, together with a disordered central Ser/Arg-rich linker. (36) Figure 2Īs mentioned above, coronavirus N protein is a multifunctional RNA-binding protein considered to be an interesting pharmacological target that merits further attention due to its critical function in viral RNA transcription and replication. (34) With regard to the search for new therapeutics based on structural proteins of SARS-CoV-2, some computational studies were carried out to identify the structure and function of E protein (35) and the other structural proteins. (33) The nucleocapsid (N) protein is a multifunctional protein that packages the viral RNA genome into a ribonucleoprotein complex called nucleocapsid to protect the genome. (32) The main function of the membrane (M) protein is to promote viral assembly due to its membrane-bending properties. (31) The envelope (E) protein is the smallest of the structural proteins but has a crucial role in assembly, budding, envelope formation, and virulence. Spike (S) protein has an important role in virus pathogenesis and organ tropism, being responsible for the viral entry through receptor recognition and membrane fusion. These proteins are less conserved than nsps, playing important functions in the viral life cycle. The club promised, "We are seriously aware of this situation and will do our best to manage players through recurrence prevention measures and continuous training.The coronavirus structural proteins that form the viral particle are the spike (S) glycoprotein, envelope (E) protein, membrane (M) protein, and the nucleocapsid (N) protein ( Figure 2). The two players said through the club, "When I was in school, I caused an irreparable wound to the victim without being aware of the severity of the violence. He added, "Sim Gyeong-seop also acknowledged that he had committed mistakes such as violently and assaulting victims during the school days of Song Lim and conveyed his apology." At that time, I confirmed that he had supported and apologized for surgical treatment." It is a situation where I conveyed my heart. The club said, "Song Myung-geun had an inappropriate conflict with the victim when he was in Songnim High School. OK Financial Group released a statement today (13th) and released a statement saying, "Song Myung-geun and Shim Gyeong-seop were involved in school violence. Men's Professional Volleyball OK Financial Group Left Song Myung-geun and Shim Gyeong-seop acknowledged that they were the perpetrators of the'school violence allegations' and apologized through the club. ▲ Song Myung-geun (left) and Shim Gyeong-seop dash to receive
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